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pt7 blue-2 t-vector  (Millipore)


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    Millipore pt7 blue-2 t-vector
    Pt7 Blue 2 T Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt7+blue-2+t-vector/pt7blue+t+vector/us07189546-310-31-38
    Average 90 stars, based on 1 article reviews
    pt7 blue-2 t-vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Nested PCR:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Amplification:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Agarose Gel Electrophoresis:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Electrophoresis:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Transfection:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Plasmid Preparation:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Polymerase Chain Reaction:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Clone Assay:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Recombinant:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    TA Cloning:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.

    Purification:

    Article Title: Double-Stranded RNA Mycovirus from Fusarium graminearum
    Article Snippet: PCR products were purified and ligated into the TOPO cloning vector (Invitrogen, Carlsbad, Calif.) or pT7 Blue-2 T-vector (Novagen, Darmstadt, Germany).

    Article Title: Polypeptide, cDNA encoding the same, and use of them
    Article Snippet: 86) was prepared additionally at 3′ end of OX003-F1 primer and then nested PCR was performed. cDNA which was amplified with clone OX003 specifically was separated with agarose-gel electrophoresis, ligated to pT7 Blue-2 T-Vector (Trade name, marketed by Novagen Co.) and transfected into E. Coli DH5a to prepare the plasmid.

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: PCR products were cloned into pT7 Blue-2 T-vector (Novagen) (as described in “Plasmid and strain construction” above).

    Article Title: Regulatory Loop between Redox Sensing of the NADH/NAD + Ratio by Rex (YdiH) and Oxidation of NADH by NADH Dehydrogenase Ndh in Bacillus subtilis
    Article Snippet: E. coli BL21(DE3)/pLysS was used to produce recombinant Rex protein with an N-terminal affinity tag of six histidines. pT7 Blue-2 T-vector (Novagen) was used for TA cloning.



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    pt7 blue-2 t-vector  (Millipore)
    90
    Millipore pt7 blue-2 t-vector
    Pt7 Blue 2 T Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt7+blue-2+t-vector/pt7blue+t+vector/us07189546-310-31-38
    Average 90 stars, based on 1 article reviews
    pt7 blue-2 t-vector - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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